ABSTRACT
Objective To study the properties of K~ +-ion channel of rat parietal cells, and to approach its effects on physiological and pathological process. Method Parietal cells were isolated. Electrophysiological records of K~ +-ion channels were made through whole cell's patch clamp technique, and the properties of the channels were analyzed. Results 10mM tetraethylammonium chloride may partially block the outwardly depolarized K~ +-ion channel current. While 10mM tetraethylammonium acetate, 10mM barium chloride or 10mM barium acetate may increases the outwardly depolarized K~ +-ion channel current. Conclusion The whole cell K~ +-ion channel current of rat parietal cells showed voltage sensitive. The current-voltage relations of whole cell potassium channels of rat parietal cells are both outwardly and inwardly rectified.
ABSTRACT
Isolation of rat gastric mucosal cells was performed with scraping method followed by protease and collagenase digestion. Parietal cells were observed and identified with phase-contrast microscopy, light microscopy with hematoxylin and eosin staining, electron microscopy, fluorescence microscopy, and also immunocytochemistry technique. The diameters of parietal cells were measured. The results showed that the mucosal cells were successfully isolated, and it was confirmed that the parietal cells were the biggest cells among all the mucosal cells.
ABSTRACT
Revised immunoelectron microscopic technique was used to examine G cells and D cells in rat antral mucosa. The number of colloidal gold granules in G and D cells was analysed through quantitative analysis. The results showed that immunological granules of colloidal gold distributed in both G and D cells. Gastrin- or somatostatin-labeled granules were present mainly as lobation-like or island-like congeries. Most of the golden congeries dissociated in the cytoplasm of G or D cells, being in the basement membrane side. A few of the golden congeries located in nucleus. The number of golden granules was 107. 04 ?19. 68 and 83. 36?17. 58 per one G and D cell, respectively.